Reference number
ISO/TS 20224-5:2020
ISO/TS 20224-5:2020
© ISO 2024
Technical Specification
ISO/TS 20224-5:2020
Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 5: Goat DNA detection method
Edition 1
2020-07
2020-07
Technical Specification
ISO/TS 20224-5:2020
ISO/TS 20224-5:2020
75478
CHF
96
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Résumé
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of goat-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of goat material derived from goat (Capra hircus).
The target sequence is a partial fragment of the goat chromosome 9 DNA sequence (i.e. GenBank accession number NC_030816.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).
Informations générales
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État actuel: PubliéeDate de publication: 2020-07Stade: Clôture de l'examen [90.60]
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Edition: 1
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Comité technique :ISO/TC 34/SC 16ICS :67.050
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Cycle de vie
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Actuellement
PubliéeISO/TS 20224-5:2020
Les normes ISO sont réexaminées tous les cinq ans
Stade: 90.60 (En cours d'examen)
